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PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFAT1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1400
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MutationContains amino acids 4-460 of NFAT1; L78P compared to WT NFAT1 (Plasmid #11100)
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Entrez GeneNfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
- Promoter CMV
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Tags
/ Fusion Proteins
- 3x HA (N terminal on backbone)
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-NFAT1(4-460)-GFP was a gift from Anjana Rao (Addgene plasmid # 11107 ; http://n2t.net/addgene:11107 ; RRID:Addgene_11107) -
For your References section:
Affinity-driven peptide selection of an NFAT inhibitor more selective than cyclosporin A. Aramburu J, Yaffe MB, Lopez-Rodriguez C, Cantley LC, Hogan PG, Rao A. Science. 1999 Sep 24. 285(5436):2129-33. 10.1126/science.285.5436.2129 PubMed 10497131