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Addgene

HA-NFAT1(4-460)-GFP
(Plasmid #11107)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11107 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFAT1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Mutation
    Contains amino acids 4-460 of NFAT1; L78P compared to WT NFAT1 (Plasmid #11100)
  • Entrez Gene
    Nfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3x HA (N terminal on backbone)
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-NFAT1(4-460)-GFP was a gift from Anjana Rao (Addgene plasmid # 11107 ; http://n2t.net/addgene:11107 ; RRID:Addgene_11107)
  • For your References section:

    Affinity-driven peptide selection of an NFAT inhibitor more selective than cyclosporin A. Aramburu J, Yaffe MB, Lopez-Rodriguez C, Cantley LC, Hogan PG, Rao A. Science. 1999 Sep 24. 285(5436):2129-33. 10.1126/science.285.5436.2129 PubMed 10497131