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Plasmid#136529PurposeTemplate for generating in situ hybridization probeDepositorInsertHouttuynia cordata BLADE-ON-PETIOLE homolog1a
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ZmaBOP1binsitu
Plasmid#136531PurposeTemplate for generating in situ hybridization probeDepositorInsertZoysia matrella BLADE-ON-PETIOLE homolog1b
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ZmaBOP2binsitu
Plasmid#136532PurposeTemplate for generating in situ hybridization probeDepositorInsertZoysia matrella BLADE-ON-PETIOLE homolog2b
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM_HcBOP1binsitu
Plasmid#136530PurposeTemplate for generating in situ hybridization probeDepositorInsertHouttuynia cordata BLADE-ON-PETIOLE homolog1b
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorUseTagsExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3_031
Plasmid#123745PurposeEncodes p38 Translocation Reporter (p38 KTR) fused to mCerulean3 as a Type 3 part to be used in the MTK systemDepositorInsertp38KTR-mCerulean3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY86
Plasmid#130949PurposeEngineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffoldDepositorInsertsgRNA-LEA2-WTmis2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 21-55 eGFP SV40
Plasmid#132653PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 41-55 eGFP SV40
Plasmid#132652PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0048
Plasmid#117611PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)DepositorInsert[35S:pEPOR1CB0012(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH90
Plasmid#128203PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH104
Plasmid#128209PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI126
Plasmid#126066PurposeModule 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI127
Plasmid#126067PurposeModule 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI177
Plasmid#126077PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP1-10
UseBinary, module 1Tagslinker (GTGGGGS)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI129
Plasmid#126210PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI161
Plasmid#126211PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJPL3[flp-6p::cmk-1::GFP]
Plasmid#124613PurposeUsed to confirm the expression of the trans-spliced GFP reporter in the expected ASE neuronsDepositorInsert[flp-6p::cmk-1::GFP]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0055
Plasmid#117622PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)DepositorInsert[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-12-C
Plasmid#124693PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only