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Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria.

Liu Y, Wan X, Wang B
Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
130907pLY9A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
130911pLY53A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
130923pLY54A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
130924pLY55A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
130925pLY56A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).
130926pLY57A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).
130927pLY59A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
130928pLY61A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
130937pLY103Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2
130938pLY108Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2
130939pLY105Engineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2
130940pLY110Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2
130941pLY106Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2
130942pLY111Engineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2
130943pLY107Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2
130944pLY112Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2
130945pLY74Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2
130946pLY124Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad
130947pLY151pspFΔHTH::λN22plus gene controlled by promoter PrhaB
130948pLY70A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
130949pLY86Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold
130950pLY152dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
130951pLY153reporter circuit (PpspA-2G6 with sfgfp::ASV)
130952pLY130Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly
130956pLY131Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly
130960pLY132Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly
130961pLY133Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly
130962pLY134Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
130963pLY76Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2
130964pLY77Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2

Antibodies from Article