pFH104
(Plasmid
#128209)
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PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTwist-Kan
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Backbone manufacturerTwist Biosciences
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Vector typePlant Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFH104 was a gift from Vladimir Nekrasov (Addgene plasmid # 128209 ; http://n2t.net/addgene:128209 ; RRID:Addgene_128209) -
For your References section:
A modular cloning toolkit for genome editing in plants. Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. 10.1186/s12870-020-02388-2 PubMed 32326901