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pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-Streptavidin-RGG

Plasmid

#242451

Purpose

Expresses the MBP-Streptavidin-RGG fusion protein in E Coli.

Depositor

Benjamin Schuster

Article

Kelley et al Nat Commun. 2025 Apr 14;16(1):3521. doi:

Insert

MBP-Streptavidin-RGG (using LAF-1 RGG domain)

Tags

MBP and Steptavidin

Expression

Bacterial

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pSKI

Plasmid

#232484

Purpose

pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

Depositor

Carlos Silva García

Article

Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

Insert

pSKI

Expression

Worm

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

RGG domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTG612

Plasmid

#237387

Purpose

mStayGold^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJW2098

Plasmid

#163094

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJW2171

Plasmid

#163095

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJW2086

Plasmid

#154335

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Expression

Worm

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMZ9

Plasmid

#198786

Purpose

Drives specific expression in the AWC (ON) neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrt-28-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ10

Plasmid

#198787

Purpose

Drives specific expression in the AWC (OFF) neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrt-47-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP005

Plasmid

#198785

Purpose

Drives specific expression in the AWB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pstr-1-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP022

Plasmid

#198798

Purpose

Drives specific expression in the PHA neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrg-13-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ41

Plasmid

#198779

Purpose

Drives specific expression in the ASH neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pnlp-76-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ43

Plasmid

#198778

Purpose

Drives specific expression in the ASH neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrj-26-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP169

Plasmid

#198769

Purpose

Drives specific expression in the ADL neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psre-1-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP063

Plasmid

#198799

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Posm-10-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge