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pCFJ90 - Pmyo-2::mCherry::unc-54utr

Plasmid

#19327

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-2::mCherry

Expression

Worm

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Expression

Worm

Promoter

rps-0

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ104 - Pmyo-3::mCherry::unc-54

Plasmid

#19328

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pJH3557

Plasmid

#191444

Purpose

Pttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Tags

wCherry

Expression

Worm

Promoter

Pttr-39

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDD346

Plasmid

#133311

Purpose

SapTrap mNG-C1 donor

Depositor

Daniel Dickinson

Insert

C. elegans codon-optimized mNeonGreen

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans codon-optimized mNeonGreen

Expression

Worm

Promoter

None

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSA102

Plasmid

#59788

Purpose

drives mCherry in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
mCherry (worm)
unc-54 3' UTR

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pJW2098

Plasmid

#163094

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ231 - Phsp Cas9

Plasmid

#191382

Purpose

Heat shock inducible Cas9 expression in C. elegans.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Phsp-16.41 Cas9 gpd-2 tagRFP-t

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.41

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP680-T2A-HO1

Plasmid

#197244

Purpose

Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP680-T2A-HO1

Expression

Worm

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJH3717

Plasmid

#191477

Purpose

Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Arch (arch-1 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-2s

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS360

Plasmid

#73687

Purpose

Punc-47::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-47::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Promoter

Punc-47

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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