lis-1::L4440
(Plasmid
#11336)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 11336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneL4440
-
Backbone manufacturerL4440 is available at Addgene
- Backbone size w/o insert (bp) 2790
-
Vector typeWorm Expression, RNAi
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namelis-1
-
SpeciesC. elegans (nematode)
-
Insert Size (bp)1200
-
GenBank IDNM_067354
-
Entrez Genelis-1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer L4440 primer (AGCGAGTCAGTGAGCGAG) (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byDr. Andrew Fire created the vector backbone L4440
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
lis-1::L4440 was a gift from Guy Caldwell (Addgene plasmid # 11336 ; http://n2t.net/addgene:11336 ; RRID:Addgene_11336)