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Addgene

lis-1::L4440
(Plasmid #11336)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11336 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    L4440
  • Backbone manufacturer
    L4440 is available at Addgene
  • Backbone size w/o insert (bp) 2790
  • Vector type
    Worm Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lis-1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1200
  • GenBank ID
    NM_067354
  • Entrez Gene
    lis-1

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Andrew Fire created the vector backbone L4440

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lis-1::L4440 was a gift from Guy Caldwell (Addgene plasmid # 11336 ; http://n2t.net/addgene:11336 ; RRID:Addgene_11336)