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Showing: 9701 - 9750 of 14291 results

  2. pJK290

    Plasmid
    #71554
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine and tyrosine-260 to …
    Promoter
    T7
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71554-plasmid-map-sequence-id-139946

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  3. pJK289

    Plasmid
    #71553
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine (please see deposito…
    Promoter
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71553-plasmid-map-sequence-id-139945

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  4. pJK326

    Plasmid
    #71557
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71557-plasmid-map-sequence-id-134622

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  5. pJK325

    Plasmid
    #71556
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71556-plasmid-map-sequence-id-131415

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  6. pJK345

    Plasmid
    #71559
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 and alanine-368
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71559-plasmid-map-sequence-id-134626

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  7. pJK344

    Plasmid
    #71558
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 (same as deleting alanine-368)
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71558-plasmid-map-sequence-id-134625

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  8. pLenti-EML4-ALK variant 3a I1171N

    Plasmid
    #183832
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N
    Promoter
    CMV
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183832-plasmid-map-sequence-id-360487

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  9. pAAV-Syn-SomaGCaMP7

    Plasmid
    #158759
    Purpose
    Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158759-plasmid-map-sequence-id-310665

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  11. pAAV-Syn-SomaGCaMP6f2

    Plasmid
    #158756
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158756-plasmid-map-sequence-id-310660

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  12. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

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  13. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has Service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158757-plasmid-map-sequence-id-310662

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  16. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  19. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid
    #158758
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    nullCoChR-GCaMP6f-Kv2.1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158758-plasmid-map-sequence-id-310664

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  21. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Use
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209086-plasmid-map-sequence-id-417159

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  22. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid
    #209085
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    209085-plasmid-map-sequence-id-416546

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  23. pGGG-Sr43

    Plasmid
    #186974
    Purpose
    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).
    Depositor
    Brande Wulff
    Article
    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:
    Insert
    Sr43
    Use
    Tags
    None
    Expression
    Bacterial and Plant
    Mutation
    None
    Promoter
    Natice
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    186974-plasmid-map-sequence-id-382711

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Showing: 9701 - 9750 of 14291 results