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Plasmid#40306PurposeA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).DepositorInsertpea APEX (Synthetic)
UseUnspecifiedTagsExpressionMutationK14D, W41F, E112K (relative to wild-type)PromoterAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Tet3
Plasmid#60940PurposeExpresses Tet3 in mammalian cellsDepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
VSV.G
Plasmid#14888DepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-RGVG-M6
Plasmid#70172PurposeExpresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNADepositorInsertRGVG-M6 T7 RNA polymerase
UseTagsHisExpressionBacterialMutationPromoterT5/LacAvailable sinceDec. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta K85A pcDNA3
Plasmid#14755DepositorInsertGSK3 beta K85A (GSK3B Human)
UseTagsHAExpressionMammalianMutationK85A. Kinase dead mutant.PromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1 CMYC
Plasmid#164145PurposeDoxycycline inducible expression of c-MYCDepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterDoxycycline inducible promoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-taCasp3-TEVp
Plasmid#45580DepositorUseAAV; Adeno associated viral vectorTagsExpressionMutationLinker replaced with a TEV protease cleavage sitePromoterEF1aAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKM468-EGFP
Plasmid#108434PurposeORBIT integrating plasmid for C-terminal tagging with EGFP.DepositorInsertattB-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX
Plasmid#167582PurposeExpress the ATP sensor GRAB_ATP1.0 in mammalian cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40
Plasmid#105540PurposeAAV expression of EGFP-Cre from hSyn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-I-SceI
Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBRPB CAG-mCherry-IP
Plasmid#106333PurposePiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.DepositorInsertmCherry-IRES-Puro
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Gal4ff.zf1
Plasmid#61392PurposemRNA synthesis of Gal4ff codon modified for zebrafishDepositorInsertGal4ff.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-C1
Plasmid#53975PurposeLocalization: C1 Cloning Vector, Excitation: 487, Emission: 509DepositorTypeEmpty backboneUseTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits