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Purposeexpresses His-tagged large fragment of Bst polymerase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET21a
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Backbone manufacturerEMD Bioscience
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 7137
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelarge fragment of Geobacillus stearothermophilus DNA polymerase I
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Alt nameBst LF
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SpeciesGeobacillus stearothermophilus
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Insert Size (bp)1776
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GenBank IDAAB52611.1
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Tag
/ Fusion Protein
- His (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.06.23.166397v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a(+)-Bst LF-6xHis was a gift from Andrea Pauli (Addgene plasmid # 159148 ; http://n2t.net/addgene:159148 ; RRID:Addgene_159148) -
For your References section:
A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing. Kellner MJ, Ross JJ, Schnabl J, Dekens MPS, Matl M, Heinen R, Grishkovskaya I, Bauer B, Stadlmann J, Menendez-Arias L, Straw AD, Fritsche-Polanz R, Traugott M, Seitz T, Zoufaly A, Fodinger M, Wenisch C, Zuber J, Pauli A, Brennecke J. Front Mol Biosci. 2022 Apr 1;9:801309. doi: 10.3389/fmolb.2022.801309. eCollection 2022. 10.3389/fmolb.2022.801309 PubMed 35433827