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Purposeexpresses His-tagged HIV reverse transcriptase and untagged HIV protease
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET21a
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Backbone manufacturerEMD Bioscience
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 7600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameHuman immunodeficiency virus 1 (HIV-1) reverse transcriptase
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Alt namerev
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Alt nameHIV RT
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SpeciesHuman immunodeficiency virus 1
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Insert Size (bp)1695
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MutationHIV-1 group M/subtype B – BH10 strain
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GenBank IDD86068.1
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Tag
/ Fusion Protein
- His (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameHuman immunodeficiency virus 1 (HIV-1) protease
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SpeciesHuman immunodeficiency virus 1
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Insert Size (bp)519
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe ORF from HIV-1 RT is based on the sequence of HIV-1 RT group M/subtype B (BH10 strain) obtained from Luis Menéndez-Arias (Spanish National Research Council, Madrid, Spain). The ORF from the HIV protease was obtained from Stuart Le Grice (National Cancer Institute, USA).
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.06.23.166397v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a(+)-HIV RT-6xHis-RBS-prot was a gift from Andrea Pauli (Addgene plasmid # 159149 ; http://n2t.net/addgene:159149 ; RRID:Addgene_159149) -
For your References section:
A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing. Kellner MJ, Ross JJ, Schnabl J, Dekens MPS, Matl M, Heinen R, Grishkovskaya I, Bauer B, Stadlmann J, Menendez-Arias L, Straw AD, Fritsche-Polanz R, Traugott M, Seitz T, Zoufaly A, Fodinger M, Wenisch C, Zuber J, Pauli A, Brennecke J. Front Mol Biosci. 2022 Apr 1;9:801309. doi: 10.3389/fmolb.2022.801309. eCollection 2022. 10.3389/fmolb.2022.801309 PubMed 35433827