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Plasmid#105122PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmTRQ2 and mVenusExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only
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pFRETgc-2in1-NC
Plasmid#105121PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmCherry and mEGFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-CN
Plasmid#105118PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmCherry and mEGFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-NN
Plasmid#105124PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmEGFP/mCherryExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-CC
Plasmid#105117PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus/tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG004
Plasmid#113979PurposeVector construction module. Level 1 golden gate cloning site module with LacZ reporterDepositorInsertLevel 1 golden gate cloning site
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGT572_Nemo
Plasmid#153487PurposeLevel 1 episomal expression plasmid for S. cerevisiaeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAP002
Plasmid#153489PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_FR13_APikL2
Plasmid#123648PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertFR13_APikL2
UseGolden gateTagsExpressionMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NC
Plasmid#105123PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus and tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-CN
Plasmid#105120PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus and tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPAP001
Plasmid#153488PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NN
Plasmid#105126PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus/tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_PGKY_APikL3
Plasmid#123652PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertPGKY_APikL3
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_CD156_APikL3
Plasmid#123651PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertCD156_APikL3
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR32_APikL2
Plasmid#123649PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR32_APikL2
UseGolden gateTagsExpressionMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR32_APikL3
Plasmid#123650PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR32_APikL3
UseGolden gateTagsExpressionMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS_1N_400
Plasmid#170476PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O1, O-, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMW#3
Plasmid#13350DepositorInsertLacZ reporter
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST5832
Plasmid#36256DepositorInsertlacZ
UseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceNov. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA3
Plasmid#46790PurposeTo express ATPase inactive Fun30 in budding yeastDepositorInsertFUN30 (FUN30 Budding Yeast)
UseTagsV5ExpressionYeastMutationATP-binding site mutated replaced lysin 603 by ar…PromoterPGAL1Available sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
SNV-memTdTomato
Plasmid#53123Purposeretroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertmemTdTomato
UseRetroviral; Expression in chicken cellsTagsExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFTK093
Plasmid#171365PurposeVector part (LVL1) from the Fungal Modular Cloning ToolKit.DepositorInsertsgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pComb3XSS
Plasmid#63890Purpose3rd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTags6x His, HA tag, and geneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pComb3XLambda
Plasmid#63892PurposeBackbone pComb3X containing PCR template for human antibody lambda light chain constant regionDepositorInserthuman antibody lambda light chain (IGLL5 Human)
UsePhage displayTagsExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterD-mannoseAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
UseTags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B4
Plasmid#31021DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B2
Plasmid#31019DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B5
Plasmid#31022DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B6
Plasmid#31023DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B3
Plasmid#31020DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B1
Plasmid#31018DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTHmut
Plasmid#200159PurposeFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purificationDepositorInsertAPOBEC1-YTHmut
UseTags6xHis, HA, and MBPExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTAG1
Plasmid#176863PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_1
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B10
Plasmid#31027DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTAG2E
Plasmid#176865PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_2E
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR29_00091361
Plasmid#121209PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR29_00091361
UseGolden gateTagsExpressionMutationMagnaporthe griseaPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A
Plasmid#31028DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A30A
Plasmid#31029DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_GY11_00131271
Plasmid#121355PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertGY11_00131271
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_FR13_00004061
Plasmid#121292PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertFR13_00004061
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B8
Plasmid#31025DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only