pPAP002
(Plasmid
#153489)
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Purpose(Empty Backbone) Level 1 episomal expression plasmid for P. pastoris
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneself made
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Vector typeYeast Expression
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Selectable markersHygromycin ; lacZ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPAP002 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153489 ; http://n2t.net/addgene:153489 ; RRID:Addgene_153489) -
For your References section:
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases. Pullmann P, Knorrscheidt A, Munch J, Palme PR, Hoehenwarter W, Marillonnet S, Alcalde M, Westermann B, Weissenborn MJ. Commun Biol. 2021 May 12;4(1):562. doi: 10.1038/s42003-021-02076-3. 10.1038/s42003-021-02076-3 PubMed 33980981