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PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSOUP (helper plasmid for all pGreen based vectors)
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Backbone manufacturerHellens et al,. 2000
- Backbone size w/o insert (bp) 9274
- Total vector size (bp) 9274
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Vector typeHelper plasmid required for replication for all pGreen based vectors
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 5 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlthough it is a high copy plasmid, it grows slightly slower that one expects. Use tetracycline at 5ug/ml.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRep A
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byProf. Roger Hellens when he was at the John Innes Centre
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000). https://doi.org/10.1023/A:1006496308160
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSOUP was a gift from John Innes Centre - Crop Transformation and Genome Editing Group & Wendy Harwood (Addgene plasmid # 165419 ; http://n2t.net/addgene:165419 ; RRID:Addgene_165419) -
For your References section:
An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat. Hayta S, Smedley MA, Clarke M, Forner M, Harwood WA. Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58. 10.1002/cpz1.58 PubMed 33656289