pFRETgc-2in1-CN
(Plasmid
#105118)
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Purpose(Empty Backbone) FRET/FLIM analysis in plants (stable or transient transformation)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFRETgc-2in1-CN
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Vector typePlant Expression
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Selectable markersBasta ; lacZ
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Tags
/ Fusion Proteins
- mCherry (N terminal on backbone)
- mGFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mCherry (N terminal on backbone)
- mGFP (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRETgc-2in1-CN was a gift from Christopher Grefen (Addgene plasmid # 105118 ; http://n2t.net/addgene:105118 ; RRID:Addgene_105118) -
For your References section:
Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies. Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C. Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. Epub 2015 May 13. 10.1104/pp.15.00533 PubMed 25971551