pFUS_B5
(Plasmid
#31022)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR8
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2447
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5a/LB+antibiotics
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLacZ + BsaI restriction sites
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Insert Size (bp)459
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AflII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer pCR8_F1 (ttgatgcctggcagttccct)
- 3′ sequencing primer pCR8_R1 (cgaaccgaacaggcttatgt) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFUS_B5 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31022 ; http://n2t.net/addgene:31022 ; RRID:Addgene_31022) -
For your References section:
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218 10.1093/nar/gkr218 PubMed 21493687