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PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNA
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKC1139
- Total vector size (bp) 10995
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Modifications to backbonemutation G8694C to remove BbsI site from pSG5 rep
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namesSpCas9
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Alt nameStreptomyces codon-optimized Cas9
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SpeciesSynthetic
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Insert Size (bp)4107
- Promoter rpsL(XC)-BbsI
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namegRNA cassette
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SpeciesSynthetic
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Insert Size (bp)538
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MutationBbsI-flanked lacZ cassette inserted in place of spacer
- Promoter gapdhp(EL)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRISPomyces-2 was a gift from Huimin Zhao (Addgene plasmid # 61737 ; http://n2t.net/addgene:61737 ; RRID:Addgene_61737) -
For your References section:
High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. Cobb RE, Wang Y, Zhao H. ACS Synth Biol. 2014 Dec 8. 10.1021/sb500351f PubMed 25458909