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Addgene

pCRISPomyces-1
(Plasmid #61736)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61736 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pKC1139
  • Total vector size (bp) 11474
  • Modifications to backbone
    mutation G9173C to remove BbsI site from pSG5 rep
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sSpCas9
  • Alt name
    Streptomyces codon-optimized Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4107
  • Promoter rpsLp(XC)-BbsI

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tracrRNA
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    86
  • Promoter rpsLp(CF)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    crRNA cassette
  • Species
    Synthetic
  • Insert Size (bp)
    523
  • Mutation
    BbsI-flanked lacZ cassette inserted in place of spacer
  • Promoter gapdhp(EL)

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPomyces-1 was a gift from Huimin Zhao (Addgene plasmid # 61736 ; http://n2t.net/addgene:61736 ; RRID:Addgene_61736)
  • For your References section:

    High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. Cobb RE, Wang Y, Zhao H. ACS Synth Biol. 2014 Dec 8. 10.1021/sb500351f PubMed 25458909