-
Plasmid#187953PurposeFKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-SpdCas9-KRAB-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA2
Plasmid#92311PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorInsertVEcad KI gRNA2 (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG
Plasmid#81023PurposeCancer-associated ASXL1 truncation mutant in retroviral vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationContains ASXL1 amino acids 1-479Promoter5’LTRAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDH2-R172K
Plasmid#87927PurposeExpresses IDH2 (R172K mutant) in mammalian cellsDepositorInsertisocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
UseTagsnoneExpressionMammalianMutationR172KPromoterCMVAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_Luciferase
Plasmid#25894DepositorInsertLuciferase
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pC001 - huLshC2C2-MBP for bacterial expression
Plasmid#79150PurposeExpresses human codon-optimized LshC2c2 for purification in E. coliDepositorInsertC2c2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP339 pGK-dCas9-SunTag
Plasmid#100943PurposedCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1aDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHAExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG
Plasmid#81022PurposeCancer-associated ASXL1 truncation mutant in retroviral vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoter5’LTRAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL
Plasmid#111389PurposeAAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsChETA-mRuby2
W3SL
UseAAVTagsmRuby2ExpressionMammalianMutationW3SL is built from a shortened WPRE, an upstream …PromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-oScarlet
Plasmid#137135PurposeViral expression of oScarletDepositorHas ServiceAAV8InsertoScarlet
UseAAVTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-3x125bp-mnx1
Plasmid#169043Purpose5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.DepositorInsertMnx1 enhancer (3x) (mnx1 Zebrafish)
UseGateway entry cloning vectorTagsExpressionMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#172196PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sitesDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_LacZ
Plasmid#25893DepositorInsertLacZ
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (1-479) 3x FLAG
Plasmid#74262PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationContains ASXL1 amino acids 1-479Promoter5’LTRAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1
Plasmid#112261PurposeDonor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.DepositorInsertspCas9-p300 (EP300 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -0
Plasmid#180007PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLOW77
Plasmid#177338PurposeC. elegans mNeonGreen co-injection markerDepositorInsertPmyo-2::mNeonGreen
UseTagsmNeonGreenExpressionWormMutationPromotermyo-2Available sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 HA-hTSPAN12
Plasmid#115784PurposeExpresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.DepositorInsertTSPAN12 (TSPAN12 Human)
UseTagsHAExpressionMammalianMutationmultiple silent mutations introduce restriction s…PromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-DsRed2
Plasmid#112219PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2DepositorInsertDsRed2
UseCRISPRTagsExpressionMammalianMutationDsRed2 contains a silent nucleotide substitution …PromoterAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2-B-SH2
Plasmid#46479DepositorInsertSH2-B (SH2B1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 515-639PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-PRDX1
Plasmid#38086DepositorInsertPRDX1 peroxiredoxin 1 (PRDX1 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-v2 RsTAL At4CL
Plasmid#126524Purposeexpresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesisDepositorInsertsUseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/human IL-8
Plasmid#17610DepositorInsertIL8 (CXCL8 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOm
Plasmid#189930PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcg 2.0-BFP-circ-arRNA backbone
Plasmid#213965Purposepcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences oDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
METTL3 shRNA 1 tet pLKO puro
Plasmid#162985PurposeTet-inducible shRNA targeting human METTL3 #1DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin
Plasmid#187954PurposeFKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-SpdCas9-hHDAC4-tagBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-MYBBP1A
Plasmid#38084DepositorInsertMYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-ACEmax
Plasmid#139105PurposeMammalian Target-ACEmax expression vector (pSI-625)DepositorInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
J23104-mRFP1-331Bb
Plasmid#78274PurposemRFP1 behind constitutive promoter J23104 in pSEVA331Bb backboneDepositorInsertJ23104-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1E335A
Plasmid#159587PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationE335APromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q
Plasmid#84349PurposeExpression of the human Huntingtin Exon1 protein containing 23Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-AnillinFL
Plasmid#187271PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full lengthDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationPromoterU6, CMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-Anillin shRNA
Plasmid#187270PurposeLentiviral expression of shRNA targeting AnillinDepositorInsertshRNA for Anillin (ANLN Human)
UseLentiviralTagsEGFPExpressionMutationPromoterU6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2
Plasmid#170273PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
UseTagsExpressionMammalianMutationSee CommentsPromoterCMVd1Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP HAT (1324-1700)
Plasmid#99340Purposebacterial expression of His tagged mouse CBP HAT domain (1324-1700)DepositorInsertmouse CBP HAT domain (1324-1700) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
TK-GFP-Nluc-P2A-neo-TK
Plasmid#134896PurposeExpresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locusDepositorInsertTK-GFP-Nluc-P2A-Neo-TK
UseCryptosporidium expressionTagsExpressionMutationPromoterCryptosporidium actin and enolaseAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Cdx2
Plasmid#128829Purposedox-dependent expression of mouse Cdx2DepositorInsertCdx2 (Cdx2 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-WT
Plasmid#45698DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -2
Plasmid#173888PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-7Q
Plasmid#84347PurposeExpression of the human Huntingtin Exon1 protein containing 7Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal His6-tagged intein SspExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZ (1079-1751)
Plasmid#99341PurposeBacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)DepositorInsertmouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAG, mCherry, and pHluorinExpressionMammalianMutationPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FPP
Plasmid#203758PurposeEncodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertHRas c-term (HRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
METTL3 shRNA 2 tet pLKO puro
Plasmid#162984PurposeTet-inducible shRNA targeting human METTL3 #2DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shSTING-Hsa
Plasmid#128158PurposeDoxycyclin inducible shRNA knockdown of human STING geneDepositorInsertshSTING Hsa (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorInsertTGFB2 (TGFB2 Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only