pFRT-DHX36iso1E335A
(Plasmid
#159587)
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PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFRT
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDHX36
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Alt nameRHAU
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Alt nameDEAH-Box Helicase 36
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Alt nameIsoform 1
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SpeciesH. sapiens (human)
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MutationE335A
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GenBank IDNM_020865.3
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Entrez GeneDHX36 (a.k.a. DDX36, G4R1, MLEL1, RHAU)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG HA (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMVf
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT-DHX36iso1E335A was a gift from Markus Hafner (Addgene plasmid # 159587 ; http://n2t.net/addgene:159587 ; RRID:Addgene_159587) -
For your References section:
DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions. Sauer M, Juranek SA, Marks J, De Magis A, Kazemier HG, Hilbig D, Benhalevy D, Wang X, Hafner M, Paeschke K. Nat Commun. 2019 Jun 3;10(1):2421. doi: 10.1038/s41467-019-10432-5. 10.1038/s41467-019-10432-5 PubMed 31160600
