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Plasmid#193212PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 geneDepositorInsertsgErich3#2 (4922501L14Rik Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cav1.3 Ca2+ channel scFv [L48A/9]
Plasmid#190515PurposeMammalian Expression of Cav1.3 Ca2+ channel scFV. Derived from hybridoma L48A/9.DepositorInsertCav1.3 Ca2+ channel (Rattus norvegicus) recombinant scFV (Cacna1d Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry
Plasmid#181854PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationHistidine 33 in DnaJB1 changed to glutamine.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-FOXI3
Plasmid#101518PurposeDonor Vector containing FOXI3 transcription factor, part of the Human TFome CollectionDepositorInsertFOXI3 (FOXI3 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]
Plasmid#136688PurposeExpresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.DepositorInsertBeclin-1 (BECN1 Human)
UseTagsTrigger FactorExpressionBacterialMutationDNA optimized for E. coli expression w/ three mut…PromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-MAN2B1-E149V
Plasmid#116426PurposeLentiviral expression of MAN2B1 E149VDepositorInsertMAN2B1 (MAN2B1 Human)
UseLentiviralTagsExpressionMutationE149VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Clcn4 [N222/6R]
Plasmid#114474PurposeMammalian Expression Plasmid of anti-Clcn4 (Human). Derived from hybridoma N222/6.DepositorInsertanti-Clcn4 (Homo sapiens) recombinant mouse monoclonal antibody (CLCN4 Mouse)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-DsRed(isoform 2)
Plasmid#99563Purposemammalian expression of TMEM230-dsRed (isoform 2)DepositorInsertTMEM230 (TMEM230 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FAM196A_p.Q46R
Plasmid#81451PurposeGateway Donor vector containing FAM196A , part of the Target Accelerator Plasmid Collection.DepositorInsertFAM196A (FAM196A Human)
UseGateway entry vectorTagsExpressionMutationQ46RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FAM196A_p.R399Q
Plasmid#81411PurposeGateway Donor vector containing FAM196A , part of the Target Accelerator Plasmid Collection.DepositorInsertFAM196A (FAM196A Human)
UseGateway entry vectorTagsExpressionMutationR399QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72924PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72617PurposeExpresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HK3 gRNA (BRDN0001146645)
Plasmid#75779Purpose3rd generation lentiviral gRNA plasmid targeting human HK3DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HK3 gRNA (BRDN0001148398)
Plasmid#75780Purpose3rd generation lentiviral gRNA plasmid targeting human HK3DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HK3 gRNA (BRDN0001149028)
Plasmid#75781Purpose3rd generation lentiviral gRNA plasmid targeting human HK3DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HK3 gRNA (BRDN0001149156)
Plasmid#75782Purpose3rd generation lentiviral gRNA plasmid targeting human HK3DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-15:ACTB-mEGFP
Plasmid#87425PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTBDepositorInsertACTB Homology Arms with mEGFP-linker (ACTB Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
TetO-FUW-OSKM
Plasmid#20321PurposeLentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OSKM
Plasmid#80481PurposepiggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-OSKM
Plasmid#20328PurposeLentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only