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  1. Lentiviral Vector Guide

    Type
    Guide
    ...vector. Science , 272 (5259), 263–267. https://doi.org/10.1126/science.272.5259.263 PMID: 8602510 Schambach...
  2. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...loxp site and one lox2272 site on either end of the transgene. Since loxp and lox2272 can not recombine...are comprised of different sequences (loxp and lox2272) and can not recombine with each other. When Cre...orientation for transcription. Upon recombination one of lox2272 sites will now be sandwiched between 2 loxp sites...binds to either pair of lox sites, let's say the lox2272, that are in the opposite orientation (direction...the loxp site is excised since it is between two lox2272 sites in the same orientation preventing the transgene...
  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... Nature methods 13.7 (2016): 557. PubMed PMID: 27240257. PubMed Central PMCID: PMC4927352 7. Hink, Mark...system." Cell 155.7 (2013): 1479-1491. PubMed PMID: 24360272. PubMed Central PMCID: PMC3918502.  14. Chernov...
  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...canonical sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant...
Showing: 1 - 20 of 54 results