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Showing: 1 - 20 of 35 results

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID: 12349826. Xiong, Wenjun, and Constance...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...loxp site and one lox2272 site on either end of the transgene. Since loxp and lox2272 can not recombine...are comprised of different sequences (loxp and lox2272) and can not recombine with each other. When Cre...orientation for transcription. Upon recombination one of lox2272 sites will now be sandwiched between 2 loxp sites...binds to either pair of lox sites, let's say the lox2272, that are in the opposite orientation (direction...the loxp site is excised since it is between two lox2272 sites in the same orientation preventing the transgene...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... Nature methods 13.7 (2016): 557. PubMed PMID: 27240257. PubMed Central PMCID: PMC4927352 7. Hink, Mark...system." Cell 155.7 (2013): 1479-1491. PubMed PMID: 24360272. PubMed Central PMCID: PMC3918502.  14. Chernov...

  10. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...2997. PubMed PMID:18658221. PubMed Central PMCID:PMC2527278. 9.Karbowski, Mariusz, et al. "Quantitation...microscopy." Biophysical journal 91.11 (2006): 4258-4272. PubMed PMID:16980368. PubMed Central PMCID:PMC1635685...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...System.” Cell 155 (2013): 1379-1491. Pubmed PMID: 24360272. PubMed Central PMCID: PMC3918502. Find plasmids...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...canonical sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant...

  18. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...Promoter-Dependent Manner. Hum Gene Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck...

  19. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...researchers all over the world. That’s an average of 27,272 plasmids distributed per year, or approximately...

  20. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ..., Gage FH, Verma IM, Trono D. Science. 1996 Apr 12;272(5259):263-7. PubMed PMID: 8602510. 2. A third-generation...
Showing: 1 - 20 of 35 results