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Plasmid#190236PurposeAAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinaseDepositorInsertEGFP
UseAAVTagsF2A, LDLR C-terminal sequence, mRFP1, myristoylat…ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TIGRE docking site targeting vector
Plasmid#61580PurposeCreate a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchangeDepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAK-2
Plasmid#210132Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAK-1
Plasmid#210131Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean
Plasmid#154951PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a1SE
Plasmid#49168PurposepHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
UseTagsmyc and pHGFP (pHIuorin)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
iYFP-Parkin Plasmid
Plasmid#197107PurposeExpresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.DepositorInsertparkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
UseRetroviralTagsYFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean
Plasmid#154948PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterCaMKIIa(0.4)Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn-somBiPOLES-mCerulean
Plasmid#154945PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-BFP
Plasmid#137129PurposeIntersectional viral expression of BFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-EYFP
Plasmid#137162PurposeIntersectional viral expression of EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-mScarlet
Plasmid#139503PurposeExpresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsmScarlet following Gly-Gly-Ser linkerExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-R1441C
Plasmid#25046DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationArginine 1441 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-oScarlet
Plasmid#137136PurposeIntersectional viral expression of oScarlet in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA pcDNA3
Plasmid#206069Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain IIDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt2-myc
Plasmid#72192PurposeExpress full-length Flrt2with a C-terminal myc tagDepositorInsertFlrt2 (Flrt2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPC1000
Plasmid#174829PurposeLentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit siteDepositorInsertsCRISPRi SpCas9 sgRNA cassette
PuroR-P2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationDetailed in manuscriptPromoterEF1a and mouse U6Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-GCaMP6F
Plasmid#137124PurposeIntersectional viral expression of GCaMP6F in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-iC++-EYFP
Plasmid#137155PurposeIntersectional viral expression of iC++-EYFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-iC++-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-GCaMP6M
Plasmid#137121PurposeIntersectional viral expression of GCaMP6M in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon/Von eYFP
Plasmid#137164PurposeIntersectional viral expression of EYFP in cells expressing Cre AND Flp AND VcreDepositorHas ServiceAAV Retrograde and AAV8Insert3x-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-iC++-EYFP
Plasmid#137157PurposeIntersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-iC++-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt1-myc
Plasmid#72191PurposeExpress full-length Flrt1 with a C-terminal myc tagDepositorInsertFlrt1 (Flrt1 Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-Y1699C
Plasmid#25048DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationTyrosine 1699 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta RING
Plasmid#133982Purposemammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of RINGPromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cav2.1 EA2, R57A, R59A pMT2
Plasmid#206122Purposeexpression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currentsDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationCav2.1-P1217fs, R57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-mCherry
Plasmid#137134PurposeIntersectional viral expression of mCherry in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-mCherry
Plasmid#137133PurposeIntersectional viral expression of mCherry in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40
Plasmid#101063PurposeAAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2_F391W-G395D
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ai95(RCL-GCaMP6f) targeting vector
Plasmid#61579PurposeTarget a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locusDepositorInsertCAG-LSL-GCaMP6-fast
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hFUS
Plasmid#50487PurposeProduces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertFUS (FUS Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTREAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-sRGECO
Plasmid#137127PurposeIntersectional viral expression of sRGECO in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-oScarlet
Plasmid#137137PurposeIntersectional viral expression of oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-BFP
Plasmid#137130PurposeIntersectional viral expression of BFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon/Von GCaMP 6m
Plasmid#137165PurposeIntersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND VcreDepositorInsert3x-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEf1aAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-sRGECO
Plasmid#137128PurposeIntersectional viral expression of sRGECO in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1
Plasmid#133978Purposemammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.CMV.CD86.C-HA
Plasmid#154848PurposeExpresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminusDepositorInsertCluster of Differentiation 86 (Cd86 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1/MIU2
Plasmid#133981Purposemammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1 and MIU2PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST53-LRRK2-K1347A
Plasmid#25052DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationLysine 1347 mutated to AlaninePromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)
Plasmid#155194PurposeExpresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminusDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTruncated sequence of THBS1 (expresses Amino Acid…PromoterCMVAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)
Plasmid#155195PurposeExpresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminusDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTHBS1 with deletion of amino acid residues 276-315PromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-ROC
Plasmid#25053DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationContains only the ROC domain- aa 1333-1516PromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX307 HRAS
Plasmid#117748PurposeOpen reading frame vector encoding HRASDepositorInsertRASH1 (HRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationClosed vector so will not read through the V5 reg…PromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU2
Plasmid#133979Purposemammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU2PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV ShadowG-AURKA-mTurq2
Plasmid#157768PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and shadowGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARMET-bio-His
Plasmid#52026PurposeExpresses full-length Mesencephalic astrocyte-derived neurotrophic factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertARMET (MANF Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only