iYFP-Parkin Plasmid
(Plasmid #197107)

• Purpose: Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.
• Depositing Lab: Dori Woods
• Publication: Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246. doi: 10.1002/adbi.202200246.

Backbone

• Vector backbone: pRetroX-TetOne Puro
• Backbone manufacturer: Takeda
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Bacterial Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: parkinson protein 2, E3 ubiquitin protein ligase
• Alt name: Parkin
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• GenBank ID: NM_004562.3
• Entrez Gene: PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
• Tag / Fusion Protein:
  • YFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: 5-TTGAATTCATGGTGAGCAAGG-3
• 3′ sequencing primer: 5-ATGGATCCTTACACGTCGAAC-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We utilized the YFP-Parkin insert from Dr. Stephen Tait's original plasmid (Plasmid #61728).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an I142V mutation in Parkinson protein 2, E3 ubiquitin protein ligase compared to the NCBI reference sequence NP_004553.2. This mutation is not expected to affect plasmid function.