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Plasmid#75982Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK10DepositorInsertMAPK10 (MAPK10 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK10 gRNA (BRDN0001148098)
Plasmid#75983Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK10DepositorInsertMAPK10 (MAPK10 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CCR5-SZ218a QTY
Plasmid#162444PurposeExpression in E coli and perform ligand binding in solutionDepositorInsertC-C chemokine receptor type 5 (CCR5 Human)
UseTagshis-tagExpressionBacterialMutationAll L, I, V, F in the transmembrane region mutate…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
CCR5-SZ190b QTY
Plasmid#162445PurposeExpression in E coli and perform ligand binding in solutionDepositorInsertC-C chemokine receptor type 5 (CCR5 Human)
UseTagshis-tagExpressionBacterialMutationAll L, I, V, F in the transmembrane region mutate…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6c
Plasmid#207853PurposeMammalian expression of PE6c prime editorDepositorInsertPE6c
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationTf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry_Atg4BC74A
Plasmid#161735PurposeDoxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagyDepositorInsertAtg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsmStrawberryExpressionMammalianMutation220-222 TGC (Cys) is changed to GCA (Ala)PromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-En1-t2a-Foxa2-hygro
Plasmid#176483PurposeTetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistanceDepositorUseTagsExpressionMammalianMutationPromoterTetOAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3056
Plasmid#144532PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHRB (THRB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.GFP
Plasmid#59404Purposeexpresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsert400 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-4:TUBA1B-mEGFP
Plasmid#87421PurposeHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1BDepositorInsertTUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-Basigin_iso2
Plasmid#221416PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertBasigin_iso2 (BSG Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Kif2c
Plasmid#208651PurposeMammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoterDepositorInsertKif2c (KIF2C Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-dnMCAK
Plasmid#208650PurposeMammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoterDepositorInsertKif2c (Kif2c Cricetulus griseus (Chinese hamster))
UseTagseGFPExpressionMammalianMutationS92A , S106A , S108A, S112A , S186A, H530A, R534A…PromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSPA8_pLX307
Plasmid#98343PurposeLentiviral expression of HSPA8DepositorInsertHSPA8 (HSPA8 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-2 pMT2
Plasmid#58732Purposeexpression of mouse Alpha2 delta-2 in mammalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMSOX17FNV
Plasmid#206382PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only