pEGFP-Kif2c
(Plasmid #208651)

• Purpose: Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter
• Depositing Lab: Samuel Bakhoum
• Publication: Li et al Nature. 2023 Aug;620(7976):1080-1088. doi: 10.1038/s41586-023-06464-z. Epub 2023 Aug 23.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: eGFP

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif2c
• Alt name: MCAK
• Species: H. sapiens (human)
• GenBank ID: NM_006845.4
• Entrez Gene: KIF2C (a.k.a. CT139, KNSL6, MCAK)
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • 208651 Li_L8P_1_pEGFP-Kif2c_pLann.gb
• A portion of this plasmid was derived from a plasmid made by: Linda Wordeman

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry