FUW-tetO-hOKMSOX17FNV
(Plasmid
#206382)
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PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFUW-tetO
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOCT4, KLF4, c-MYC, SOX17FNV
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Alt nameOCT4-T2A-KLF4-P2A-c-MYC-E2A-SOX17FNV
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SpeciesH. sapiens (human)
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
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Entrez GeneSOX17 (a.k.a. VUR3)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUW-tetO-hOKMSOX17FNV was a gift from Ralf Jauch (Addgene plasmid # 206382 ; http://n2t.net/addgene:206382 ; RRID:Addgene_206382) -
For your References section:
Evaluation of the determinants for improved pluripotency induction and maintenance by engineered SOX17. Hu H, Ho DHH, Tan DS, MacCarthy CM, Yu CH, Weng M, Scholer HR, Jauch R. Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597. 10.1093/nar/gkad597 PubMed 37607832
