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Addgene

pLPS-hSox2-gfp
(Plasmid #49390)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49390 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLPS-3'EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression, Cre/Lox ; acceptor vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox2
  • Alt name
    human Sry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    953
  • Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
  • GenBank ID
    BC013923
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
When the recombinant construct is expressed in mammalian cells, EGFP is expressed from the immediate early promoter of cytomegalovirus (pCMV IE) as a 3' fusion to Sox2, through intron splicing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPS-hSox2-gfp was a gift from Randall McKinnon (Addgene plasmid # 49390 ; http://n2t.net/addgene:49390 ; RRID:Addgene_49390)