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pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Tags

Expression

Mutation

Promoter

unknown

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSG5 PPAR alpha

Plasmid

#22751

Depositor

Bruce Spiegelman

Insert

PPARalpha (Ppara Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hChR2(H134R)-mCherry

Plasmid

#26976

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

Depositor

Karl Deisseroth

Has Service

AAV5, AAV8, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Beta1-N-18

Plasmid

#55064

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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HA-p62

Plasmid

#28027

Purpose

Mammalian expression of HA tagged human p62

Depositor

Qing Zhong

Article

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

p62 (SQSTM1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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MPZ(P0)promoter pGL3-TATA

Plasmid

#21259

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero promoter (Mpz Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-SOX4

Plasmid

#51304

Purpose

Expression of SOX4 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

SRY (sex determining region Y)-box 4 (SOX4 Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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FUmGW

Plasmid

#22479

Depositor

Connie Cepko

Article

Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

membrane-bound green fluorescent protein

Use

Lentiviral

Tags

palmitoylation

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Myc3-Nrf2

Plasmid

#21555

Purpose

Mammalian expression of human Nrf2 with Myc tag

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP-Rab5

Plasmid

#14437

Purpose

Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Article

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 5 (RAB5A Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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Ubiquitin WT

Plasmid

#12647

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

Ubiquitin - wild type (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pBAD-mTagBFP2

Plasmid

#34632

Depositor

Vladislav Verkhusha

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

mTagBFP2

Use

Tags

Expression

Bacterial

Mutation

I174A compared to mTagBFP (enhanced photostabilit…

Promoter

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-TRAF6-wt

Plasmid

#21624

Depositor

John Kyriakis

Article

Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

Insert

TNF receptor-associated factor 6 (Traf6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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MXS_BidirectionalCAG

Plasmid

#78175

Purpose

Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

Bidirectional CAG Promoter

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAS9counter

Plasmid

#107192

Purpose

Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

Cas9
sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

prab17

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5B

Plasmid

#61802

Purpose

Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

RAB5B (RAB5B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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