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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Backbone manufacturerI. Verma, Salk
- Backbone size w/o insert (bp) 9165
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemembrane-bound green fluorescent protein
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Alt namemGFP
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Alt nameGFP
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SpeciesAequorea victoria
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Insert Size (bp)799
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Tag
/ Fusion Protein
- palmitoylation (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI, BamHI, AgeI, (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUmGW was a gift from Connie Cepko (Addgene plasmid # 22479 ; http://n2t.net/addgene:22479 ; RRID:Addgene_22479) -
For your References section:
Retinal progenitor cells can produce restricted subsets of horizontal cells. Rompani SB, Cepko CL. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7. 10.1073/pnas.0709979104 PubMed 18162542