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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPARalpha
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Alt namePPAR alpha
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Alt namePPARa
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SpeciesM. musculus (mouse)
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Entrez GenePpara (a.k.a. 4933429D07Rik, Nr1c1, PPAR-alpha, PPARalpha, Ppar)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The PPARa cDNA was isolated from an adipocyte lambda ZAPII cDNA library (Tontonoz et al 1994 Genes Dev 8:1224-1234).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5 PPAR alpha was a gift from Bruce Spiegelman (Addgene plasmid # 22751 ; http://n2t.net/addgene:22751 ; RRID:Addgene_22751)