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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSV-Sport
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3160
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPAR gamma
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Alt namePPAR gamma 1
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Alt namePPARg1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1800
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GenBank ID
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Entrez GenePparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PPARg1 is missing the first 30aa of PPARg2. See figures 4 and 5 of the associated publication for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSV Sport PPAR gamma 1 was a gift from Bruce Spiegelman (Addgene plasmid # 8886 ; http://n2t.net/addgene:8886 ; RRID:Addgene_8886) -
For your References section:
mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM. Genes Dev. 1994 May 15. 8(10):1224-34. 10.1101/gad.8.10.1224 PubMed 7926726