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Addgene

pSV Sport PPAR gamma 2
(Plasmid #8862)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8862 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSV-Sport
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3160
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR gamma
  • Alt name
    PPAR gamma 2
  • Alt name
    PPARg2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • GenBank ID
    NM_011146
  • Entrez Gene
    Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XhoI/HindIII (destroyed during cloning)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV Sport PPAR gamma 2 was a gift from Bruce Spiegelman (Addgene plasmid # 8862 ; http://n2t.net/addgene:8862 ; RRID:Addgene_8862)
  • For your References section:

    mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM. Genes Dev. 1994 May 15. 8(10):1224-34. 10.1101/gad.8.10.1224 PubMed 7926726