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Plasmid#75085Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCDNA3.1- dCas9-2xNLS-EGFP
Plasmid#74710PurposeExpresses dCas9-2xNLS-EGFP in mammalian cellsDepositorInsertdCas9-2xNLS-EGFP
UseCRISPRTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0574
Plasmid#143714PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXA13 (HOXA13 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_sU6
Plasmid#69351PurposesgRNA scaffold and synthetic U6 promoterDepositorInsertsU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotersynthetic U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HDM-SARS2-Spike-delta21
Plasmid#155130PurposeEncodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotypingDepositorInsertSARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationCodon optimized to H. sapiens using IDT codon opt…PromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-acc(3)IV
Plasmid#69862PurposepCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Myr.PKCalpha.FLAG
Plasmid#10807DepositorInsertPKC alpha (PRKCA Bovine)
UseTagsFLAG and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMVhTRIM65_3Flag
Plasmid#53966PurposeHuman TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus.DepositorInsertTRIM65 (TRIM65 Human)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
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MSCV-LMP1-IRES-mCherry
Plasmid#220351PurposeExpresses EBV latent gene LMP1 in mammalian cellsDepositorInsertLMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
LB701: pMVP/PB/mCherry-DEST
Plasmid#121871PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Lck-tdTomato
Plasmid#118739PurposeTo express membrane targeted tdTomatoDepositorInsertLck domain (LCK Human)
UseLentiviralTagstdTomatoExpressionMutationH8N (see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP43
Plasmid#78953PurposeFigure 2. Expresses BxbI.DepositorInsertBxbI
UseTagsExpressionMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR' CMV GFP
Plasmid#14858DepositorInsertCMV-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSII423
Plasmid#35464DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
PX458-AAVS1
Plasmid#113194PurposeEncodes gRNA for human AAVS1DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMVSP6-nEGFP-SV40-PURO
Plasmid#138364PurposeLentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selectionDepositorInsertnuclear EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
mVenus-P115-C1
Plasmid#112931Purposep115RhoGEF tagged with mVenus for detection of Ga13 activityDepositorInsertp115 RhoGEF
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1346 U6-B2M sgRNA Gag-pol v2
Plasmid#201917PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
UseTagsExpressionMammalianMutationPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig2
Plasmid#64842PurposeA lentiviral vector for the expression of mouse Olig2 under doxycyline controlDepositorInsertOlig2 (Olig2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SFFV-GLuc-CreERT2-mCherry
Plasmid#178185Purposethe plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.DepositorInsertsGLuc
CreERT2
mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Gal4ff.zf1
Plasmid#61392PurposemRNA synthesis of Gal4ff codon modified for zebrafishDepositorInsertGal4ff.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-hIFITM3-Y20A
Plasmid#58462PurposeExpresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-EGFP
Plasmid#174186PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertEGFP with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18
Plasmid#131670PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.DepositorInserte18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag beta-1-adrenergic-receptor
Plasmid#14698DepositorInsertB1AR (ADRB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Akaluc (neo)
Plasmid#124701PurposeAka-Luciferase reporterDepositorInsertVenus-Akaluciferase
UseLentiviralTagsExpressionMutationPromoterhPGKAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L1-Puro
Plasmid#181917PurposeLentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MBP-2xNLS-tdTomato
Plasmid#104054PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoterDepositorInsert2xNLS-tdTomato
UseAAVTagsNLSExpressionMutationPromoterMBPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_dTAG-KANSL3-targeting
Plasmid#170755PurposeDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locusDepositorInsertBSD-P2A-2xHA-dTAG
UseMouse TargetingTagsBSD-P2A-2xHA-dTAGExpressionMutationPhenylalanine 36 to valine in FKBP12PromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-his-BACH1 WT
Plasmid#17642DepositorInsertBACH1 (BRIP1 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Letm1
Plasmid#191962PurposeExpression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).DepositorInsertLetm1 (LETM1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Lyso-B
Plasmid#209873PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP B
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
R-pre-mRFP
Plasmid#17275DepositorInsertR-pre
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
c-myc-PT3EF1a
Plasmid#92046PurposeExpresses c-Myc in mammalian cellsDepositorInsertC-MYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceJuly 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV OMM-FKBP-V5-sTurboID (N)
Plasmid#153006PurposeExpresses split-TurboID N-terminal fragment targeted to outer mitochondrial membraneDepositorInsertOMM (Tom20)-sTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-iATPSnFR1.0
Plasmid#102553Purposeexpresses cytoplasmic ATP sensor in astrocytesDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWAP-HGF
Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingTagsExpressionMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PHyTc Notch4HA
Plasmid#21469DepositorInsertNotch4 (Notch4 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-NDP52
Plasmid#175749PurposeLentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-EWSR1
Plasmid#46384DepositorInsertEWSR1 (EWSR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorInsertKDR (KDR Human)
UseTagsLabeled with mCherry on the C-Terminus via a 3 am…ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mSTING
Plasmid#214156PurposeTransient expression of STINGDepositorInsertSTING (Sting1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only