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A high-performance genetically encoded fluorescent biosensor for imaging physiological peroxynitrite.

Chen Z, Zhang S, Li X, Ai HW
Cell Chem Biol. 2021 Jan 27. pii: S2451-9456(21)00013-1. doi: 10.1016/j.chembiol.2021.01.013. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
157923pBAD-pnGFP-UltraPeroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)
157924pMAH-pnGFP-UltraPeroxynitrite sensor pnGFP-Ultra in mammalian cells (use with pMAH-POLY-eRF1(E55D))
157925pMAH-POLY-eRF1(E55D)Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Antibodies from Article