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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10807 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6
- Backbone size w/o insert (bp) 4650
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKC alpha
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Alt namePKC-A
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Alt namePRKCA
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SpeciesB. taurus (bovine)
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Insert Size (bp)2300
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GenBank IDNM_174435
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Entrez GenePRKCA (a.k.a. PKRCA)
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Tags
/ Fusion Proteins
- Myr (N terminal on insert)
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constitutively active. Myr sequence from p60src.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myr.PKCalpha.FLAG was a gift from Alex Toker (Addgene plasmid # 10807 ; http://n2t.net/addgene:10807 ; RRID:Addgene_10807) -
For your References section:
Effect of overexpression of protein kinase C alpha on rat lacrimal gland protein secretion. Hodges RR, Kazlauskas A, Toker A, Dartt DA. Adv Exp Med Biol. 2002 . 506(Pt A):237-41. PubMed 12613914
Map uploaded by the depositor.