pHaMDRwt
(Plasmid #10957)

• Depositing Lab: Michael Gottesman
• Publication: Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(12):4486-90.

Backbone

• Vector backbone: pHa
• Backbone size w/o insert (bp): 11000
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MDR1
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• GenBank ID: M14758
• Entrez Gene: ABCB1 (a.k.a. ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 32 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines.

Figure legend for author's map:
Structure of retroviral vector carrying MDR] cDNA.
sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho
I. bp, Base pairs; amp, ampicillin resistance gene.