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Plasmid#51592Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 308 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S241A
Plasmid#51591Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 241 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
UAS-dar1-V5
Plasmid#45056DepositorInsertdar1 (dar1 Fly)
UseTagsV5ExpressionInsectMutationPromoterHS promoterAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
CJ50 (cg11094 utr mut)
Plasmid#17778DepositorInsertcg11094 utr fragment mut
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationmutant versionPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ58 (cg6551 utr mut)
Plasmid#17784DepositorInsertcg6551 utr fragment mut
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationmutant versionPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT277
Plasmid#122608Purposeexpress evoCDA1-BE4max in mammalian cellsDepositorInsertevoCDA1-BE4max
UseTagsExpressionMammalianMutationF23S A123V I195FPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-nlsCRE
Plasmid#12265DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_TRPM8
Plasmid#64879PurposeExpresses Ion Channel TRPM8 fused to eGFP in mammalian cellsDepositorInsertTransient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
UseTagseGFPExpressionMutationPromoterAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fNPY-GFP
Plasmid#22912DepositorInsertfugu neuropeptide Y promoter driving GFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-GfaABC1D-GRAB_ATP1.0
Plasmid#167579PurposeExpress the ATP sensor GRAB_ATP1.0 in astrocytesDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX
Plasmid#167582PurposeExpress the ATP sensor GRAB_ATP1.0 in mammalian cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX307 Luciferase
Plasmid#117734PurposeOpen reading frame vector encoding LuciferaseDepositorInsertfirefly luciferase
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-EF1α-nCas9/RT
Plasmid#210188PurposeExpression of nCas9/RT-P2A-PuroDepositorInsertnCas9/RT-P2A-Puro
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2/pAAV
Plasmid#91718PurposeAAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKIIDepositorInsertmEGFP-P2A-paAIP2
UseAAVTagsExpressionMutationPromoterCaMKII 1.3Available sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-hSyn-EGFP
Plasmid#114215PurposeLentiviral vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-EGFP-ADRP
Plasmid#87157PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromotermCherry-R TTGGTCACCTTCAGCTTGGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
wtTDP43tdTOMATOHA
Plasmid#28205DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsHA tag and tdtomatoExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX
Plasmid#167583PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cellsDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES RED/SP M87C448Y
Plasmid#89324PurposeExpression of RFP and M87C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 deletedPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-OTp-mCherry
Plasmid#184754PurposeTo drive mCherry under the control of mouse oxytocin promoterDepositorInsertmCherry
UseAAVTagsExpressionMutationPromotermouse Oxytocin promoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-empty
Plasmid#105594Purposeempty control vector with GFP marker and puro resistanceDepositorInsertno insert, empty control vector
UseRetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT120b
Plasmid#122604PurposeBE PACE selection, express gIII, luxAB and degron-GTCCA guide RNADepositorInsertgIII, luxAB, guide RNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#122600PurposeBE PACE selection, express C-intein-Cas9-ugi fusionDepositorInsertintein-dCas9-ugi fusion
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_ATP1.0
Plasmid#167581PurposeExpress the ATP sensor GRAB_ATP1.0 in Cre positive cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitC
Plasmid#122605Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT100.106b
Plasmid#122601Purposeclone APOBEC1 phage by SapI Golden GateDepositorInsertAPOBEC1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Apex2-Venus-CAAX
Plasmid#168511PurposeExpression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.DepositorInsertApex2
UseTagsVenus-CAAXExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neuronsDepositorInsertsmGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-cyto-GCaMP6f
Plasmid#52925PurposeFluorescent reporter for calcium signalingDepositorHas ServiceAAV5InsertGCaMP6f
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_ATP1.0mut
Plasmid#167580PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytesDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
FU- dio PSD95-mCherry- W
Plasmid#73919PurposeExpresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.DepositorInsertPSD-95-mCherry
UseLentiviralTagsmCherry and mycExpressionMammalianMutationPromoterUbi-CAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCherry-mNrp1
Plasmid#21934DepositorInsertneuropilin 1 (Nrp1 Mouse)
UseTagsCherry and HisExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBT372
Plasmid#125613PurposeExpresses evoCDA1-BE4max-NG in mammalian cellsDepositorInsertevoCDA1-BE4max-NG
UseTagsExpressionMammalianMutationF23S A123V I195FPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-PALB2
Plasmid#71114Purposemammalian expession of FLAG-PALB2DepositorInsertPALB2 (PALB2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoExpressionMutationPromoterCAGAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (no Cre)
Plasmid#200370PurposeBackbone plasmid for cloning HiUGE GS-gRNA (without Cre)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- SIV-MAC251 vpx
Plasmid#115810PurposeEncodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance proteinDepositorInsertSIVMAC251 Vpx
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-dsRed-shscramble
Plasmid#71384PurposeExpresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cellsDepositorInsertCMV promoter-dsRed-mir30-shscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterCMV enhancer/promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBT2dR3-proB
Plasmid#122595PurposeBE PACE selection, express T7 RNA polymerase with degron-GTCCA targetDepositorInsertT7 RNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX307 hcRed1
Plasmid#117732PurposeOpen reading frame vector encoding hcRed1DepositorInserthcRed1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (hSyn-Cre) backbone
Plasmid#200368PurposeBackbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only