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PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neurons
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71761 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4941
- Total vector size (bp) 7404
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namemGFP
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Insert Size (bp)777
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Tag
/ Fusion Protein
- Palmitoylation signal (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameSynaptophysin
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Insert Size (bp)951
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Tag
/ Fusion Protein
- mRuby (C terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby was a gift from Liqun Luo (Addgene plasmid # 71761 ; http://n2t.net/addgene:71761 ; RRID:Addgene_71761) -
For your References section:
Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic Input-Output Mapping. Beier KT, Steinberg EE, DeLoach KE, Xie S, Miyamichi K, Schwarz L, Gao XJ, Kremer EJ, Malenka RC, Luo L. Cell. 2015 Jul 30;162(3):622-34. doi: 10.1016/j.cell.2015.07.015. 10.1016/j.cell.2015.07.015 PubMed 26232228
Map uploaded by the depositor.