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pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

Plasmid

#171027

Purpose

Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1 and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

ChrimsonR is fused to tdTomato

Expression

Mutation

Promoter

Ef1a

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP(long)-FLEX-GCaMP7f

Plasmid

#189612

Purpose

Cre-dependent GCaMP7f under the GFAP promoter

Depositor

Martin Riccomagno

Article

Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

Insert

GCaMP7f

Use

AAV and Cre/Lox

Tags

GCaMP7f

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pattB-LSL-AAVR-F2A-spCas9

Plasmid

#202459

Purpose

The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

Depositor

Jan Carette

Article

Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

Insert

AAVR (AU040320 Mouse)

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

mCherry fusion

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-Cas9C-P2A-turboGFP

Plasmid

#80942

Purpose

Expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2n-WPRE

Plasmid

#218875

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

Synapsin

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChETA-EYFP

Plasmid

#26968

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Has Service

AAV1, AAV5, and AAV9

Insert

hChR2(E123T/H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T, H134R

Promoter

EF1alpha

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[CoChR-GFP]

Plasmid

#62724

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-CAG-Flpe-ERT2

Plasmid

#68460

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-tdTomato-WPRE-pGHpA

Plasmid

#67527

Purpose

"Expresses the fluorescent protein tdTomato"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

tandem dimer tomato

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-mScarlet-WPRE

Plasmid

#99280

Purpose

Can be used to generate AAV virus that will express mScarlet in the presence of Cre

Depositor

Rylan Larsen

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ASAP3Kv-WPRE

Plasmid

#132330

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3KV

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-EYFP-WPRE

Plasmid

#154870

Purpose

Human synapsin-1 promoter; Flp-dependent EYFP

Depositor

Ulrik Gether

Insert

EYFP

Use

AAV and Mouse Targeting; Flp-frt

Tags

Expression

Mammalian

Mutation

Promoter

Human synapsin promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-DIO-VGLUT2:miniSOG

Plasmid

#214200

Purpose

For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

Depositor

Thomas Hnasko

Article

Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

Insert

Slc17a6 tagged with miniSOG and hemagglutinin

Use

AAV and Cre/Lox

Tags

miniSOG (tagged with HA) is fused to the first in…

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FRT-EF1a-TVA-2A-N2cG

Plasmid

#172361

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-NeoR-CAG-EGFPnls-WPRE

Plasmid

#191692

Purpose

For knocking pCAG-EGFPnls into AAVS1 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

AAVS1 homology arms and pCAG-EGFPnls

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-oChIEF-tdTomato

Plasmid

#30541

Purpose

Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

Depositor

Rory McQuiston

Article

Bell et al Neuropharmacology. 2011 Aug 25.

Insert

oChIEF-tdtomato

Use

AAV and Cre/Lox

Tags

tdTomato

Expression

Mutation

Promoter

CAG

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-3xGFP

Plasmid

#191204

Purpose

FlpO dependent GFP expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-BFP

Plasmid

#137131

Purpose

Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-OMOR-eYFP

Plasmid

#66847

Purpose

Expresses Opto-MOR in a Cre dependent manner

Depositor

Michael Bruchas

Article

Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

Insert

Opto Mu opioid receptor

Use

AAV and Cre/Lox

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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