pGP-AAV-syn-iGABASnFR2n-WPRE
(Plasmid
#218875)
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PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-Syn
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiGABASnFR2n
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Alt namevariant 4445
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SpeciesPseudomonas fluorescens, A. victoria (jellyfish)
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Insert Size (bp)1941
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MutationS99A F104H R168P
- Promoter Synapsin
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-AAV-syn-iGABASnFR2n-WPRE was a gift from GENIE Project (Addgene plasmid # 218875 ; http://n2t.net/addgene:218875 ; RRID:Addgene_218875)