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Plasmid#189926PurposeExpresses nuclear-localized mCherry-XL (R125K) in mammalian cellsDepositorInsertmCherry-XL (R125K)
UseTagsH2BExpressionMammalianMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoterCMV7Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP
Plasmid#11179PurposeMammalian expression vector for expression of CFPDepositorInsertECFP from Aequorea victoria
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_sfCherry2(1-10)
Plasmid#82602PurposeExpresses sfCherry2(1-10) fusion in mammalian cellsDepositorInsertsfCherry2(1-10)
UseTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
ACBE-Q
Plasmid#204608PurposeMammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-QDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationTadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW62.YY1.FKBP.knock-in.mCherry
Plasmid#104370PurposeFor knocking in FKBP F36V P2A mCherry onto YY1DepositorInsertFKBP12(F36V) -P2A-mCherry
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CD9-10
Plasmid#55013PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorInsertCD9 (CD9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
CN4253-rAAV-3xCore2_eHGT_380h-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#208165PurposeDirect-expressing SYFP2 in astrocytes AAV VirusInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)
Plasmid#140534PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2DepositorInsertOsTIR1(F74G) mAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
UseTagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_EBOV
Plasmid#103052PurposeEncodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertL gene of Ebola virus (L Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS6
Plasmid#55752PurposeProduces T7 RNA Polymerase.DepositorInsertT7 RNA Polymerase
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_EBOV
Plasmid#103051PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-iSeroSnFR
Plasmid#128484PurposeFluorescent reporter for serotonin (mammalian expression, membrane localization tag)DepositorInsertiSeroSnFR
UseTagsIg-kappa leader, Myc, PDGFR + enhanced ER export,…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRC0940 (circRNA entry vector)
Plasmid#189967PurposeCloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestionDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
NG-ABEmax
Plasmid#124163PurposeA-to-G base editorDepositorInsertTadA-TadA(evo)-Cas9-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)
Plasmid#108292PurposepX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta wt pcDNA3
Plasmid#14753PurposeMammalian expression of human GSK3 beta with HA tagDepositorInsertGSK3 beta (GSK3B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiGuide Cherry
Plasmid#170510PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinaseDepositorInsertFlpe
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:NLuc
Plasmid#118065PurposeTranscriptional unit for constitutive expression of NLuc in mammalian cellsDepositorInsertCMV:NLuc:bGH
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB_Hygro
Plasmid#192663Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance markerDepositorInsertdCas9-KRAB
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFG4
Plasmid#87251Purposegene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-HphDepositorInsertHyg
UseTagsparS segmentsExpressionYeastMutationPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-TNNT2-Neo
Plasmid#214013PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Merlin
Plasmid#84293PurposeExpression of wt MerlinDepositorInsertMerlin (NF2) (NF2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-BMTU UDG-6xHis
Plasmid#172197Purposeexpresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactionsDepositorInsertBMTU UDG
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)
Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
piRFP670-N1
Plasmid#45457DepositorHas ServiceCloning Grade DNAInsertiRFP670
UseTagsExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-pcDNA3.1 Puro-CAG-Voltron2-ST
Plasmid#172910PurposeMammalian expression of voltage sensor; soma localization targeting sequenceDepositorInsertVoltron2-ST
UseTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterCAGAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.BB.3z
Plasmid#200679PurposeModular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ub-G76V-GFP
Plasmid#11941DepositorInsertUb-G76V-GFP
UseTagsExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
GST-HA-GFP11-N1
Plasmid#91953PurposeExpresses GST-HA-GFP11 in mammalian cellsDepositorInsertGST-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ND4-DdCBE-right side TALE
Plasmid#157843Purposeexpresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIP403
Plasmid#122028PurposePlasmid for expression of gene of interest in Lactobacillus plantarum and L. sakeiDepositorInsertgusA
UseTagsExpressionBacterialMutationPromotersppAAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only