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Plasmid#78827PurposeTo purify GST-tagged recombinant RIPK3 proteinDepositorInsertRIPK3 (RIPK3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
UseTagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…PromoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
PclpB GFP pZa
Plasmid#170074PurposeGFP expression under the control of E. coli clpB promoterDepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSG310
Plasmid#115611Purposesurface-displayed Ag2, Arabinose-inducible; medium-copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-TT (TetR, P21)
Plasmid#45989DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-150
Plasmid#118564PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG/Q157TAG
Plasmid#68299PurposeE17/Q157 TAG GFP reporterDepositorInsertGFP E17TAG/Q157TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRCP
Plasmid#195862PurposeEncodes chromogenic protein (CP). CP inducible with rhamnose.DepositorInsertChromogenic Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRCPam
Plasmid#195861PurposeEncodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.DepositorInsertAmber Chromogenic Protein on pRCPam plasmid
UseTagsExpressionBacterialMutationGln62XPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET-AtC60αβC20NtL
Plasmid#160876PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC051-HisMS2_PLP_pac_LC
Plasmid#128234PurposeLower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE2-TetOminiCMV-OS
Plasmid#136576PurposeDox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2DepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Bcatfl
Plasmid#24193DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pACM4-ACA
Plasmid#49809PurposeEncodes fabD, accA, accB, accC, accD in pseudo-operon configuration.DepositorInsertsACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase
UseTagsExpressionBacterialMutationSilent site-directed mutagenesis to remove SalI s…PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only