A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
68283 | SepOTSβ | Phosphoserine orthogonal translation system |
68284 | SepOTSγ | Phosphoserine orthogonal translation system |
68285 | SepOTSδ | Phosphoserine orthogonal translation system |
68286 | SepOTSε | Phosphoserine orthogonal translation system |
68287 | SepOTSζ | Phosphoserine orthogonal translation system |
68288 | SepOTSη | Phosphoserine orthogonal translation system |
68289 | SepOTSθ | Phosphoserine orthogonal translation system |
68290 | SepOTSι | Phosphoserine orthogonal translation system |
68291 | SepOTSκ | Phosphoserine orthogonal translation system |
68292 | SepOTSλ | Phosphoserine orthogonal translation system (preferred variant) |
68294 | SepOTSν | Phosphoserine orthogonal translation system |
68295 | GFP E17TAG | E17TAG GFP reporter |
68296 | GFP S2TAG | S2TAG GFP reporter |
68297 | GFP Q157TAG | Q157TAG GFP reporter |
68298 | GFP S2TAG/E17TAG | S2TAG/E17TAG GFP reporter |
68299 | GFP E17TAG/Q157TAG | E17/Q157 TAG GFP reporter |
68300 | MBP-MEK1 | N-terminal MBP tagged human MEK1 gene for bacterial expression |
68301 | MBP-MEK1 S218TAG | N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression |
68302 | MBP-MEK1 S222TAG | N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression |
68305 | Beta lactamase S68TAG | Beta lactamase S68TAG gene |
68306 | Strain: C321.ΔA | Genomically recoded strain of E. coli (no genomic TAG) |
69118 | E17TAG GFP zeo resistance | For E17TAG GFP reporter expression in non-zeocin resistant strains |
198598 | SupD v2 | Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine. |