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Plasmid#12260Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)DepositorTypeEmpty backboneUseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only
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pMD2.G
Plasmid#12259PurposeVSV-G envelope expressing plasmidDepositorInsertVSV G
UseLentiviral; EnvelopeTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7
Plasmid#214812PurposeMammalian expression of SpCas9 PE7 prime editorDepositorInsertPE7
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-VSV-G
Plasmid#8454PurposeEnvelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1
Plasmid#41393Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZT-C13-R1
Plasmid#62197PurposeChr.13/CLYBL TALEN expression vectorDepositorInsertRight CLYBL TALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZT-C13-L1
Plasmid#62196PurposeChr.13/CLYBL TALEN expression vectorDepositorInsertLeft CLYBL TALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+Lux
Plasmid#26159DepositorInsertBacterial luciferase operon
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-Mito-7
Plasmid#56018PurposeLocalization: Mitochondria, Excitation: 440, Emission: 620DepositorInsertMito (COX8A Human)
UseTagsmKeima-RedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRGFP
Plasmid#26475PurposeIn vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection markerDepositorInsertDRGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUMVC
Plasmid#8449PurposePackaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
PB533-42B3mutAC2-sfGFP
Plasmid#186777PurposeRNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cellsDepositorInsert42B3mutAC2-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP
Plasmid#20672PurposeDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEVS104
Plasmid#207393PurposeConjugal helper plasmidDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti dCAS-VP64_Blast
Plasmid#61425Purpose3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)DepositorHas ServiceLentiviral PrepInsertdCAS9(D10A, N863A)-VP64_2A_Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only