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Plasmid#200297PurposeBacterial expression plasmids for production of recombinant Pseudomonas fluorescens Q2-87 MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PaODH
Plasmid#110802PurposeExpresses PaODH and opine dehydrogenase from Pseudomonas aeruginosaDepositorInsertPa4835
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOE7771.1
Plasmid#135077PurposeMannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vectorDepositorInsertmtlR
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPaGE Pyl TAG FliC T248TAG
Plasmid#160089PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.DepositorInsertsPyrrolysyl tRNA(cua) (Methanosarcina mazei)
Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Flagellin
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Threonine 248 to TAG stop-codon.PromoterPseudomonas aeruginosa Leu-tRNA native promoter a…Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PaNAS
Plasmid#110801PurposeExpresses PaNAS nicotianamine synthase from pseudomonas aeruginosaDepositorInsertPa4836
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b- pchB WT
Plasmid#70099Purposeexpresses isochorismate-pyruvate lyase from Pseudomonas aeruginosaDepositorInsertpchB
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pvdA WT
Plasmid#87098Purposeexpresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosaDepositorInsertpvdA WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCPP5741
Plasmid#128722PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcV-hopV1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchA WT
Plasmid#87102Purposeexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosaDepositorInsertpchA WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
PfICH pET15b
Plasmid#60684PurposeRecombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescensDepositorInsertinhA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
Stag-PaMT
Plasmid#215160PurposeEncodes a Pseudomonas aeruginosa metallothionein fused with the S-tag at the N-terminusDepositorInsertStag-PaMT
UseTagsS-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR
Plasmid#113348PurposeA sgRNA expression plasmid for genome editing in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pnCasPA-BEC
Plasmid#113349PurposeA cytidine deaminase-mediated base-editing plasmid in Pseudomonas speciesDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCPP5238
Plasmid#128711PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopG1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5052
Plasmid#128729PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAM1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5537
Plasmid#128730PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only