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Addgene

pCPP5741
(Plasmid #128722)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128722 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pENTR/SD/D-TOPO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shcV-hopV1
  • Species
    Pseudomonas syringae

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC identified a D145G in shcV compared to the reference sequence that the depositing lab has not found to be of functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCPP5741 was a gift from Alan Collmer (Addgene plasmid # 128722 ; http://n2t.net/addgene:128722 ; RRID:Addgene_128722)
  • For your References section:

    Modular Study of the Type III Effector Repertoire in Pseudomonas syringae pv. tomato DC3000 Reveals a Matrix of Effector Interplay in Pathogenesis. Wei HL, Zhang W, Collmer A. Cell Rep. 2018 May 8;23(6):1630-1638. doi: 10.1016/j.celrep.2018.04.037. 10.1016/j.celrep.2018.04.037 PubMed 29742421