pCPP5741
(Plasmid
#128722)
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PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/SD/D-TOPO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshcV-hopV1
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SpeciesPseudomonas syringae
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC identified a D145G in shcV compared to the reference sequence that the depositing lab has not found to be of functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCPP5741 was a gift from Alan Collmer (Addgene plasmid # 128722 ; http://n2t.net/addgene:128722 ; RRID:Addgene_128722) -
For your References section:
Modular Study of the Type III Effector Repertoire in Pseudomonas syringae pv. tomato DC3000 Reveals a Matrix of Effector Interplay in Pathogenesis. Wei HL, Zhang W, Collmer A. Cell Rep. 2018 May 8;23(6):1630-1638. doi: 10.1016/j.celrep.2018.04.037. 10.1016/j.celrep.2018.04.037 PubMed 29742421