PflQ2MT Pseudomonas fluorescens Q2-87 Metallothionein
(Plasmid
#200297)
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PurposeBacterial expression plasmids for production of recombinant Pseudomonas fluorescens Q2-87 Metallothionein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTYB21
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGene of metallothionein with Chiting Binding Domanin-Intein
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SpeciesPseudomonas fluorescens
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Tag
/ Fusion Protein
- Chiting Binding Domanin-Intein (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PflQ2MT Pseudomonas fluorescens Q2-87 Metallothionein was a gift from Artur Krężel (Addgene plasmid # 200297 ; http://n2t.net/addgene:200297 ; RRID:Addgene_200297) -
For your References section:
Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn. Mosna K, Jurczak K, Krezel A. Metallomics. 2023 Oct 4;15(10):mfad061. doi: 10.1093/mtomcs/mfad061. 10.1093/mtomcs/mfad061 PubMed 37804185