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Addgene

pET28b-PaNAS
(Plasmid #110801)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110801 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6170
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pa4836
  • Alt name
    PaNAS - Nicotianamine Synthase
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    801
  • GenBank ID
    NP_253523.1
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pET Upstream
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was synthesized by Genscript.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-PaNAS was a gift from Audrey Lamb (Addgene plasmid # 110801 ; http://n2t.net/addgene:110801 ; RRID:Addgene_110801)
  • For your References section:

    Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa. McFarlane JS, Lamb AL. Biochemistry. 2017 Nov 14;56(45):5967-5971. doi: 10.1021/acs.biochem.7b00804. Epub 2017 Nov 3. 10.1021/acs.biochem.7b00804 PubMed 29091735