pET28b-PaNAS
(Plasmid
#110801)
-
PurposeExpresses PaNAS nicotianamine synthase from pseudomonas aeruginosa
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 110801 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET28b
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6170
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePa4836
-
Alt namePaNAS - Nicotianamine Synthase
-
SpeciesPseudomonas aeruginosa PAO1
-
Insert Size (bp)801
-
GenBank IDNP_253523.1
- Promoter T7
-
Tag
/ Fusion Protein
- Hexahistidine tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pET Upstream
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byGene was synthesized by Genscript.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET28b-PaNAS was a gift from Audrey Lamb (Addgene plasmid # 110801 ; http://n2t.net/addgene:110801 ; RRID:Addgene_110801) -
For your References section:
Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa. McFarlane JS, Lamb AL. Biochemistry. 2017 Nov 14;56(45):5967-5971. doi: 10.1021/acs.biochem.7b00804. Epub 2017 Nov 3. 10.1021/acs.biochem.7b00804 PubMed 29091735