pET29b- pchB WT
(Plasmid
#70099)
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Purposeexpresses isochorismate-pyruvate lyase from Pseudomonas aeruginosa
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5370
- Total vector size (bp) 5613
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepchB
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SpeciesPseudomonas aeruginosa PAO1
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Insert Size (bp)306
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GenBank IDGCA_000006765.1
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer 5-TGG CGT ATC ATA TGA AAA CTC CGA A-3
- 3′ sequencing primer 5-TTT AGA AAG CTT TGC GGC ACC CCG-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b- pchB WT was a gift from Audrey Lamb (Addgene plasmid # 70099 ; http://n2t.net/addgene:70099 ; RRID:Addgene_70099) -
For your References section:
Two crystal structures of the isochorismate pyruvate lyase from Pseudomonas aeruginosa. Zaitseva J, Lu J, Olechoski KL, Lamb AL. J Biol Chem. 2006 Nov 3;281(44):33441-9. Epub 2006 Aug 16. 10.1074/jbc.M605470200 PubMed 16914555