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Addgene

pET29b- pchB WT
(Plasmid #70099)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70099 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 5613
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pchB
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    306
  • GenBank ID
    GCA_000006765.1
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer 5-TGG CGT ATC ATA TGA AAA CTC CGA A-3
  • 3′ sequencing primer 5-TTT AGA AAG CTT TGC GGC ACC CCG-3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b- pchB WT was a gift from Audrey Lamb (Addgene plasmid # 70099 ; http://n2t.net/addgene:70099 ; RRID:Addgene_70099)
  • For your References section:

    Two crystal structures of the isochorismate pyruvate lyase from Pseudomonas aeruginosa. Zaitseva J, Lu J, Olechoski KL, Lamb AL. J Biol Chem. 2006 Nov 3;281(44):33441-9. Epub 2006 Aug 16. 10.1074/jbc.M605470200 PubMed 16914555
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